Reporter
psb1C3_int
Part:BBa_K743016:Design
Designed by: Juan Simón Álamos Group: iGEM12_UC_Chile (2012-09-22)
psb1C3_IntKR recombination plasmid with RFP under psbA2 promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2551
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2551
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2551
Illegal XhoI site found at 3048 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2551
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2551
Illegal AgeI site found at 1164
Illegal AgeI site found at 1276 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 511
Design Notes
This composite part is not intended to be used for standard biobrick assembly because of a EcoR1 restriction site in psb1C3_intK plasmid. You should use gibson assembly.
Source
psbA2 was amplified from Synechocystis genomic DNA and its sequence was obtained from BBa_K390017. RFP is from BBa_E1010.
For more information about the plasmid backbone, check psb1C3_IntKR recombination plasmid page.